Composite
A2

Part:BBa_K4927044:Design

Designed by: Chuan Liu   Group: iGEM23_HUBU-SKY-China   (2023-10-06)


A2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You need to note that the label is connected to the N-terminal or C-terminal of methanol dehydrogenase, as this can cause changes in the activity of methanol dehydrogenase.After our testing, we found that when the label is connected to the C-terminal, the activity of methanol dehydrogenase is better.


Source

Methanol dehydrogenase is derived from Stearothermophilus, and the CL2 label was designed by our team.

References